Journal: European Journal of Clinical Investigation
Article Title: FNDC4 and FNDC5 Attenuate SARS‐CoV‐2 S1‐Induced Inflammatory Responses in Human Adipose Tissue
doi: 10.1111/eci.70215
Figure Lengend Snippet: Co‐localisation of FNDC4 and FNDC5 with ACE2‐expressing macrophage populations in adipose tissue. Double immunofluorescence showing co‐localisation of pan‐macrophage marker CD68 ( green ) with (A) ACE2, (B) FNDC4 or (C) FNDC5 ( red ) in sections of visceral (VAT) (C) and subcutaneous (SAT) adipose tissue; nuclei were stained with DAPI ( blue ) (magnification 200×). Representative images of at least three separate experiments are shown. (D) Immunohistochemical staining for CD68, ACE2, FNDC4 and FNDC5 in paired VAT (G) and SAT (H) serial sections (magnification ×200) ( n = 6).
Article Snippet: After washing, coverslips were mounted using Vectashield with DAPI (H‐1500‐10, Vector Laboratories, Burlingame, CA, USA) for nuclear counterstaining.
Techniques: Expressing, Immunofluorescence, Marker, Staining, Immunohistochemical staining